LOCUS PWY-5046_T1 4779 bp DNA linear SYN 30-JUL-2026 DEFINITION Synthetic construct PWY-5046_T1; designed by SynPAL design pipeline. ACCESSION PWY-5046_T1 VERSION PWY-5046_T1 KEYWORDS SynPAL; synthetic construct. SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. FEATURES Location/Qualifiers source 1..4779 /organism="synthetic construct" /mol_type="other DNA" /note="designed by SynPAL design pipeline" misc_feature 1..40 /note="flanking_up: upstream regulatory flank [40,800]bp" /note="source organism: Bacillus subtilis subtilis 168" promoter 1..40 /note="placeholder promoter (native upstream flank)" gene 41..1465 /gene="lpdV" /locus_tag="lpdV" CDS 41..1465 /gene="lpdV" /locus_tag="lpdV" /organism="Bacillus subtilis subtilis 168" /note="native genomic strand -" gene 1466..2458 /gene="bkdAA" /locus_tag="bkdAA" CDS 1466..2458 /gene="bkdAA" /locus_tag="bkdAA" /organism="Bacillus subtilis subtilis 168" /note="native genomic strand -" gene 2459..3442 /gene="bkdAB" /locus_tag="bkdAB" CDS 2459..3442 /gene="bkdAB" /locus_tag="bkdAB" /organism="Bacillus subtilis subtilis 168" /note="native genomic strand -" gene 3443..4717 /gene="bkdB" /locus_tag="bkdB" CDS 3443..4717 /gene="bkdB" /locus_tag="bkdB" /organism="Bacillus subtilis subtilis 168" /note="native genomic strand -" misc_feature 4718..4779 /note="flanking_down: downstream regulatory flank [40,800]bp" /note="source organism: Bacillus subtilis subtilis 168" terminator 4718..4779 /note="placeholder terminator (native downstream flank)" ORIGIN 1 aacaatatcg tattgaatag acagacagga gtgagtcacc atggcaactg agtatgacgt 61 agtcattctg ggcggcggta ccggcggtta tgttgcggcc atcagagccg ctcagctcgg 121 cttaaaaaca gccgttgtgg aaaaggaaaa actcggggga acatgtctgc ataaaggctg 181 tatcccgagt aaagcgctgc ttagaagcgc agaggtatac cggacagctc gtgaagccga 241 tcaattcgga gtggaaacgg ctggcgtgtc cctcaacttt gaaaaagtgc agcagcgtaa 301 gcaagccgtt gttgataagc ttgcagcggg tgtaaatcat ttaatgaaaa aaggaaaaat 361 tgacgtgtac accggatatg gacgtatcct tggaccgtca atcttctctc cgctgccggg 421 aacaatttct gttgagcggg gaaatggcga agaaaatgac atgctgatcc cgaaacaagt 481 gatcattgca acaggatcaa gaccgagaat gcttccgggt cttgaagtgg acggtaagtc 541 tgtactgact tcagatgagg cgctccaaat ggaggagctg ccacagtcaa tcatcattgt 601 cggcggaggg gttatcggta tcgaatgggc gtctatgctt catgattttg gcgttaaggt 661 aacggttatt gaatacgcgg atcgcatatt gccgactgaa gatctagaga tttcaaaaga 721 aatggaaagt cttcttaaga aaaaaggcat ccagttcata acaggggcaa aagtgctgcc 781 tgacacaatg acaaaaacat cagacgatat cagcatacaa gcggaaaaag acggagaaac 841 cgttacctat tctgctgaga aaatgcttgt ttccatcggc agacaggcaa atatcgaagg 901 catcggccta gagaacaccg atattgttac tgaaaatggc atgatttcag tcaatgaaag 961 ctgccaaacg aaggaatctc atatttatgc aatcggagac gtaatcggtg gcctgcagtt 1021 agctcacgtt gcttcacatg agggaattat tgctgttgag cattttgcag gtctcaatcc 1081 gcatccgctt gatccgacgc ttgtgccgaa gtgcatttac tcaagccctg aagctgccag 1141 tgtcggctta accgaagacg aagcaaaggc gaacgggcat aatgtcaaaa tcggcaagtt 1201 cccatttatg gcgattggaa aagcgcttgt atacggtgaa agcgacggtt ttgtcaaaat 1261 cgtggctgac cgagatacag atgatattct cggcgttcat atgattggcc cgcatgtcac 1321 cgacatgatt tctgaagcgg gtcttgccaa agtgctggac gcaacaccgt gggaggtcgg 1381 gcaaacgatt cacccgcatc caacgctttc tgaagcaatt ggagaagctg cgcttgccgc 1441 agatggcaaa gccattcatt tttaaatgag tacaaaccga catcaagcac tagggctgac 1501 tgatcaggaa gccgttgata tgtatagaac catgctgtta gcaagaaaaa tcgatgaaag 1561 aatgtggctg ttaaaccgtt ctggcaaaat tccatttgta atctcttgtc aaggacagga 1621 agcagcacag gtaggagcgg ctttcgcact tgaccgtgaa atggattatg tattgccgta 1681 ctacagagac atgggtgtcg tgctcgcgtt tggcatgaca gcaaaggact taatgatgtc 1741 cgggtttgca aaagcagcag atccgaactc aggaggccgc cagatgccgg gacatttcgg 1801 acaaaagaaa aaccgcattg tgacgggatc atctccggtt acaacgcaag tgccgcacgc 1861 agtcggtatt gcgcttgcgg gacgtatgga gaaaaaggat atcgcagcct ttgttacatt 1921 cggggaaggg tcttcaaacc aaggcgattt ccatgaaggg gcaaactttg ccgctgtcca 1981 taagctgccg gttattttca tgtgtgaaaa caacaaatac gcaatctcag tgccttacga 2041 taagcaagtc gcatgtgaga acatttccga ccgtgccata ggctatggga tgcctggcgt 2101 aactgtgaat ggaaatgatc cgctggaagt ttatcaagcg gttaaagaag cacgcgaaag 2161 ggcacgcaga ggagaaggcc cgacattaat tgaaacgatt tcttaccgcc ttacaccaca 2221 ttccagtgat gacgatgaca gcagctacag aggccgtgaa gaagtagagg aagcgaaaaa 2281 aagtgatccc ctgcttactt atcaagctta cttaaaggaa acaggcctgc tgtccgatga 2341 gatagaacaa accatgctgg atgaaattat ggcaatcgta aatgaagcga cggatgaagc 2401 ggagaacgcc ccatatgcag ctcctgagtc agcgcttgat tatgtttatg cgaagtagat 2461 gtcagtaatg tcatatattg atgcaatcaa tttggcgatg aaagaagaaa tggaacgaga 2521 ttctcgcgtt ttcgtccttg gggaagatgt aggaagaaaa ggcggtgtgt ttaaagcgac 2581 agcgggactc tatgaacaat ttggggaaga gcgcgttatg gatacgccgc ttgctgaatc 2641 tgcaatcgca ggagtcggta tcggagcggc aatgtacgga atgagaccga ttgctgaaat 2701 gcagtttgct gatttcatta tgccggcagt caaccaaatt atttctgaag cggctaaaat 2761 ccgctaccgc agcaacaatg actggagctg tccgattgtc gtcagagcgc catacggcgg 2821 aggcgtgcac ggagccctgt atcattctca atcagtcgaa gcaattttcg ccaaccagcc 2881 cggactgaaa attgtcatgc catcaacacc atatgacgcg aaagggctct taaaagccgc 2941 agttcgtgac gaagaccccg tgctgttttt tgagcacaag cgggcatacc gtctgataaa 3001 gggcgaggtt ccggctgatg attatgtcct gccaatcggc aaggcggacg taaaaaggga 3061 aggcgacgac atcacagtga tcacatacgg cctgtgtgtc cacttcgcct tacaagctgc 3121 agaacgtctc gaaaaagatg gcatttcagc gcatgtggtg gatttaagaa cagtttaccc 3181 gcttgataaa gaagccatca tcgaagctgc gtccaaaact ggaaaggttc ttttggtcac 3241 agaagataca aaagaaggca gcatcatgag cgaagtagcc gcaattatat ccgagcattg 3301 tctgttcgac ttagacgcgc cgatcaaacg gcttgcaggt cctgatattc cggctatgcc 3361 ttatgcgccg acaatggaaa aatactttat ggtcaaccct gataaagtgg aagcggcgat 3421 gagagaatta gcggagtttt aaatggcaat tgaacaaatg acgatgccgc agcttggaga 3481 aagcgtaaca gaggggacga tcagcaaatg gcttgtcgcc cccggtgata aagtgaacaa 3541 atacgatccg atcgcggaag tcatgacaga taaggtaaat gcagaggttc cgtcttcttt 3601 tactggtacg ataacagagc ttgtgggaga agaaggccaa accctgcaag tcggagaaat 3661 gatttgcaaa attgaaacag aaggcgcgaa tccggctgaa caaaaacaag aacagccagc 3721 agcatcagaa gccgctgaga accctgttgc aaaaagtgct ggagcagccg atcagcccaa 3781 taaaaagcgc tactcgccag ctgttctccg tttggccgga gagcacggca ttgacctcga 3841 tcaagtgaca ggaactggtg ccggcgggcg catcacacga aaagatattc agcgcttaat 3901 tgaaacaggc ggcgtgcaag aacagaatcc tgaggagctg aaaacagcag ctcctgcacc 3961 gaagtctgca tcaaaacctg agccaaaaga agagacgtca tatcctgcgt ctgcagccgg 4021 tgataaagaa atccctgtca caggtgtaag aaaagcaatt gcttccaata tgaagcgaag 4081 caaaacagaa attccgcatg cttggacgat gatggaagtc gacgtcacaa atatggttgc 4141 atatcgcaac agtataaaag attcttttaa gaagacagaa ggctttaatt taacgttctt 4201 cgcctttttt gtaaaagcgg tcgctcaggc gttaaaagaa ttcccgcaaa tgaatagcat 4261 gtgggcgggg gacaaaatta ttcagaaaaa ggatatcaat atttcaattg cagttgccac 4321 agaggattct ttatttgttc cggtgattaa aaacgctgat gaaaaaacaa ttaaaggcat 4381 tgcgaaagac attaccggcc tagctaaaaa agtaagagac ggaaaactca ctgcagatga 4441 catgcaggga ggcacgttta ccgtcaacaa cacaggttcg ttcgggtctg ttcagtcgat 4501 gggcattatc aactaccctc aggctgcgat tcttcaagta gaatccatcg tcaaacgccc 4561 ggttgtcatg gacaatggca tgattgctgt cagagacatg gttaatctgt gcctgtcatt 4621 agatcacaga gtgcttgacg gtctcgtgtg cggacgattc ctcggacgag tgaaacaaat 4681 tttagaatcg attgacgaga agacatctgt ttactaaata agcaaaaaga gcattttttg 4741 aagttttgtt tcaaaaaatg ctctttttct atgctttat //